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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9139849 B1

PDFFull Text
Date of Patent:
Sep. 22, 2015

Filed:

Apr. 07, 2006

Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation

Applicants:

Vladimir L. Larionov, Potomac, MD (US);

William C. Earnshaw, Cingletie Peeples, GB;

Reto Gassman, Zurich, CH;

Stefanie Kandels-lewis, Dielheim-Balzfeld, DE;

Hiroshi Masumoto, Rockville, MD (US);

Megumi Nakano, Rockville, MD (US);

Vladimir Noskov, Rockville, MD (US);

Natalay Y. Kouprina, Potomac, MD (US);

Carl J. Barrett, Chapel Hill, NC (US);

Stefano Cardinale, Edinburgh, GB;

Inventors:

Vladimir L. Larionov, Potomac, MD (US);

William C. Earnshaw, Cingletie Peeples, GB;

Reto Gassman, Zurich, CH;

Stefanie Kandels-Lewis, Dielheim-Balzfeld, DE;

Hiroshi Masumoto, Rockville, MD (US);

Megumi Nakano, Rockville, MD (US);

Vladimir Noskov, Rockville, MD (US);

Natalay Y. Kouprina, Potomac, MD (US);

Carl J. Barrett, Chapel Hill, NC (US);

Stefano Cardinale, Edinburgh, GB;

Assignees:

The United States of America as Represented by the Government of the Department of Health and Human Services, Washington, DC (US);

The University Court of the University of Edinburgh, Edinburg, GB;

Attorney:

Klarquist Sparkman, LLP

Primary Examiner:

Kenneth R Horlick

Assistant Examiner:

Joyce Tung

Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 2800/204 (2013.01); C12N 2800/206 (2013.01); C12N 2800/208 (2013.01); C12N 2830/003 (2013.01);
Abstract

Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure. Artificial chromosomes, including human artificial chromosomes with a regulated centromere, and methods of their use are also provided.

Find Patent Forward Citations

Larionov, Vladimir. (2015). Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation (U.S. Patent No. 9139849). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9139849

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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