The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 01, 2015
Filed:
May. 18, 2012
Stephen Douglas Barrett, Hartland, MI (US);
Daniel Austin Bochar, Ann Arbor, MI (US);
Levi Lynn Blazer, Ann Arbor, MI (US);
Fred Lawrence Ciske, Dexter, MI (US);
Gregory William Endres, Saline, MI (US);
Jeffrey Keith Johnson, Ann Arbor, MI (US);
Gregory Scott Keyes, Dexter, MI (US);
Ranjinder Singh Sidhu, Ann Arbor, MI (US);
Raymond C. Trievel, Ypsilanti, MI (US);
Margaret Lynn Collins, Canton, MI (US);
Stephen Douglas Barrett, Hartland, MI (US);
Daniel Austin Bochar, Ann Arbor, MI (US);
Levi Lynn Blazer, Ann Arbor, MI (US);
Fred Lawrence Ciske, Dexter, MI (US);
Gregory William Endres, Saline, MI (US);
Jeffrey Keith Johnson, Ann Arbor, MI (US);
Gregory Scott Keyes, Dexter, MI (US);
Ranjinder Singh Sidhu, Ann Arbor, MI (US);
Raymond C. Trievel, Ypsilanti, MI (US);
Margaret Lynn Collins, Canton, MI (US);
CAYMAN CHEMICAL COMPANY, INCORPORATED, Ann Arbor, MI (US);
THE REGENTS OF THE UNIVERSITY OF MICHIGAN, Ann Arbor, MI (US);
Abstract
Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM -utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.