The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 25, 2015

Filed:

Nov. 15, 2011
Applicants:

Eva Albers, Partille, SE;

Lisbeth Olsson, Göteborg, SE;

Rakesh Koppram, Göteborg, SE;

Inventors:

Eva Albers, Partille, SE;

Lisbeth Olsson, Göteborg, SE;

Rakesh Koppram, Göteborg, SE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/12 (2006.01); C12P 7/06 (2006.01); C12P 7/10 (2006.01); C12N 9/04 (2006.01); C12N 15/01 (2006.01); C12N 15/81 (2006.01); C12Q 1/04 (2006.01); C12N 9/12 (2006.01); C12R 1/865 (2006.01);
U.S. Cl.
CPC ...
C12P 7/06 (2013.01); C12N 9/0006 (2013.01); C12N 9/1205 (2013.01); C12N 15/01 (2013.01); C12N 15/81 (2013.01); C12P 7/10 (2013.01); C12Q 1/04 (2013.01); C12R 1/865 (2013.01); C12Y 101/0101 (2013.01); C12Y 101/01009 (2013.01); C12Y 101/01307 (2013.01); C12Y 207/01017 (2013.01); Y02E 50/16 (2013.01); Y02E 50/17 (2013.01);
Abstract

A method for producing a strain ofwith introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation, improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains ofobtained by the method according to the invention are described.


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