The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2015

Filed:

Mar. 30, 2012
Applicants:

Luca Lanzano, Irvine, CA (US);

Michelle Digman, Irvine, CA (US);

Enrico Gratton, San Clemente, CA (US);

Inventors:

Luca Lanzano, Irvine, CA (US);

Michelle Digman, Irvine, CA (US);

Enrico Gratton, San Clemente, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01C 3/08 (2006.01); G02B 21/00 (2006.01); G01B 9/04 (2006.01); G01N 21/64 (2006.01); B82Y 35/00 (2011.01);
U.S. Cl.
CPC ...
G02B 21/0012 (2013.01); G01B 9/04 (2013.01); G01N 21/6458 (2013.01); B82Y 35/00 (2013.01); G02B 2207/113 (2013.01);
Abstract

An optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved three dimensional images of very small and moving features in live cells and in a matter of seconds. Images of microvilli in live cultured opossum kidney cells expressing NaPi co-transporter proteins with different GFP constructs and images of cell protrusions in a collagen matrix are produced with a resolution of about 20 nm. Along cell protrusions in three dimensional cellular adhesions could be identified to the extracellular matrix.


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