The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 2015

Filed:

Jun. 25, 2009
Applicants:

Michael Okura, Honolulu, HI (US);

Bruce J. Richardson, Los Gatos, CA (US);

Inventors:

Michael Okura, Honolulu, HI (US);

Bruce J. Richardson, Los Gatos, CA (US);

Assignee:

Emergence Genomics, LLC, Honolulu, HI (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); C12Q 1/68 (2006.01); B01L 7/00 (2006.01); C40B 60/12 (2006.01); G01N 21/03 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6408 (2013.01); B01L 7/00 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); B01J 2219/00529 (2013.01); B01J 2219/00722 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/088 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1838 (2013.01); B01L 2400/0487 (2013.01); C12Q 1/68 (2013.01); G01N 21/0332 (2013.01); G01N 2021/6441 (2013.01);
Abstract

A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector.


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