The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 2015
Filed:
Jul. 23, 2014
Research and Diagnostic Systems, Inc., Minneapolis, MN (US);
Zhengliang L. Wu, Edina, MN (US);
Research & Diagnostics Systems, Inc., Minneapolis, MN (US);
Abstract
A method of detecting sulfotransferase activity including conducting a first reaction comprising, measuring free phosphate produced by the first reaction, and comparing the measured free phosphate to a free phosphate standard curve or equation or to a free phosphate level obtained in a separate reaction. The first reaction includes combining the sulfotransferase, Golgi-resident PAP-phosphatase (gPAPP), a 5'-nucleotidase, a substrate of the sulfotransferase, and 3′-phosphoadenosine-5′-phosphosulfate (PAPS) under conditions to produce 3′-phosphoadenosine-5′-phosphate (PAP).