The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 2015
Filed:
Dec. 22, 2009
Hiroshi Tahara, Osaka, JP;
Yusuke Suzuki, Osaka, JP;
Keiichi Yamamoto, Osaka, JP;
Yuzuru Kitahara, Osaka, JP;
Yasuhiko Suzuki, Sapporo, JP;
Hiroshi Tahara, Osaka, JP;
Yusuke Suzuki, Osaka, JP;
Keiichi Yamamoto, Osaka, JP;
Yuzuru Kitahara, Osaka, JP;
Yasuhiko Suzuki, Sapporo, JP;
National University Corporation Hokkaido University, Hokkaido, JP;
Fuso Pharmaceutical Industries, Ltd., Osaka, JP;
Abstract
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.