The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2015

Filed:

May. 27, 2011
Applicants:

Rolf Schmid, Stuttgart, DE;

Michael Braun, Bad Mergentheim-Edelfingen, DE;

Luo Liu, Beijing, CN;

Arno Aigner, Tuntenhausen, DE;

Dirk Weuster-botz, München, DE;

Inventors:

Rolf Schmid, Stuttgart, DE;

Michael Braun, Bad Mergentheim-Edelfingen, DE;

Luo Liu, Beijing, CN;

Arno Aigner, Tuntenhausen, DE;

Dirk Weuster-Botz, München, DE;

Assignee:

Pharmazell GmbH, Raubling, DE;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 33/00 (2006.01); C12N 9/04 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C12N 15/87 (2006.01); C07K 14/245 (2006.01); C12P 33/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0006 (2013.01); C07K 14/245 (2013.01); C12P 33/00 (2013.01); C12P 33/06 (2013.01); C12Y 101/01159 (2013.01);
Abstract

The invention relates to 12 α-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 α-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 α-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12α-hydroxysteroids using said 12α-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 α-hydroxysteroid dehydrogenases.


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