The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 21, 2015

Filed:

Sep. 09, 2011
Applicants:

Asha A. Oroskar, Oak Brook, IL (US);

John P. Lacava, New York, NY (US);

Michael Paul Rout, New York, NY (US);

Anil R. Oroskar, Oak Brook, IL (US);

Inventors:

Asha A. Oroskar, Oak Brook, IL (US);

John P. LaCava, New York, NY (US);

Michael Paul Rout, New York, NY (US);

Anil R. Oroskar, Oak Brook, IL (US);

Assignees:

OROCHEM Technologies, Inc., Naperville, IL (US);

The Rockefeller University, New York, NY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 30/02 (2006.01); B01L 3/00 (2006.01); G01N 33/68 (2006.01); G01N 35/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/50255 (2013.01); G01N 33/6803 (2013.01); B01L 2200/0631 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0487 (2013.01); G01N 35/00 (2013.01);
Abstract

This invention relates to a kit and a method for the collection and analysis of complex protein mixtures. More particularly, the invention relates to a kit comprising a single barrel filtration well or a multi-well filtration plate wherein each well comprises an upper filtration zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper filtration zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone. The upper filtration zone comprises an upper collection zone, a sponge zone, and a deep bed filtration zone; and, the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane and a lower bed filtration media. When used with an array of selected buffer solutions, the multi-well filtration plate can provide accurate, automated, high-throughput protein analysis by affinity chromatography.


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