The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 07, 2015
Filed:
May. 21, 2012
Megan Anderson, Washington, DC (US);
Peilin Chen, Richmond, CA (US);
Brian Fowler, San Mateo, CA (US);
Robert C. Jones, Los Altos, CA (US);
Fiona Kaper, Solano Beach, CA (US);
Ronald Lebofsky, Etobicoke, CA;
Andrew May, San Francisco, CA (US);
Megan Anderson, Washington, DC (US);
Peilin Chen, Richmond, CA (US);
Brian Fowler, San Mateo, CA (US);
Robert C. Jones, Los Altos, CA (US);
Fiona Kaper, Solano Beach, CA (US);
Ronald Lebofsky, Etobicoke, CA;
Andrew May, San Francisco, CA (US);
Fluidigm Corporation, South San Francisco, CA (US);
Abstract
Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.