The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 30, 2015

Filed:

May. 12, 2009
Applicants:

Robin Coope, Vancouver, CA;

Daniel Horspool, Montreal, CA;

Robert A. Holt, North Vancouver, CA;

Inventors:

Robin Coope, Vancouver, CA;

Daniel Horspool, Montreal, CA;

Robert A. Holt, North Vancouver, CA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C07H 21/00 (2013.01); C12Q 1/6846 (2013.01); C12N 15/10 (2013.01); C12N 15/66 (2013.01);
Abstract

The invention provides a system and method for synthesizing polynucleotides by solid phase assembly oligonucleotide precursors, in accordance with the method, a polynucleotide is partitioned into an ordered set of subunits, wherein each subunit is assembled in a single reaction from a subset of oligonucleotide precursors that uniquely anneal together to produce the subunit. The subunits are then assembled to form the desired polynucleotide. An important feature of the invention is the selection of subunits that are free of undesired sequence elements, such as palindromes, repetitive sequences, and the like, which would result in more than one subunit product alter ligating a pool of oligonucleotide precursors.


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