The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 02, 2015
Filed:
Jun. 05, 2012
Werner Hoelke, Penzberg, DE;
Johann-peter Thalhofer, Weilheim, DE;
Antje Liehre, Penzberg, DE;
Markus Weber, Habach, DE;
Werner Hoelke, Penzberg, DE;
Johann-Peter Thalhofer, Weilheim, DE;
Antje Liehre, Penzberg, DE;
Markus Weber, Habach, DE;
Roche Diagnostic Operations, Inc., Indianapolis, IN (US);
Abstract
The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilizate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.