The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 2015
Filed:
May. 12, 2014
Applicant:
Crucell Holland B.v., Leiden, NL;
Inventor:
John A. Lewis, Little Compton, RI (US);
Assignee:
Crucell Holland B.V., Leiden, NL;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); A61K 39/13 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); A61K 39/13 (2013.01); C12N 2770/32634 (2013.01); C12N 2770/32651 (2013.01);
Abstract
Described is a process for producing poliovirus, the process comprising: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×10cells/ml and 150×10cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.