The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

Feb. 29, 2008
Applicants:

Denis Drapeau, Salem, NH (US);

Jessica Snow, Sterling, MA (US);

Gregory Hiller, Wakefield, MA (US);

Yen Tung Luan, Chelmsford, MA (US);

Inventors:

Denis Drapeau, Salem, NH (US);

Jessica Snow, Sterling, MA (US);

Gregory Hiller, Wakefield, MA (US);

Yen Tung Luan, Chelmsford, MA (US);

Assignee:

Wyeth LLC, Madison, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0018 (2013.01); C12N 2500/20 (2013.01); C12N 2500/32 (2013.01); C12N 2510/02 (2013.01);
Abstract

An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.


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