The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2015

Filed:

Aug. 26, 2005
Applicants:

Patricia L. Clark, South Bend, IN (US);

Michael S. Evans, South Bend, IN (US);

Krastyu G. Ugrinov, Notre Dame, IN (US);

Thomas Clarke, Iv, Notre Dame, IN (US);

Marc-andré Frese, Enger, DE;

Inventors:

Patricia L. Clark, South Bend, IN (US);

Michael S. Evans, South Bend, IN (US);

Krastyu G. Ugrinov, Notre Dame, IN (US);

Thomas Clarke, IV, Notre Dame, IN (US);

Marc-André Frese, Enger, DE;

Assignee:

Other;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/04 (2006.01); C12N 15/00 (2006.01); C40B 30/04 (2006.01);
U.S. Cl.
CPC ...
C40B 30/04 (2013.01);
Abstract

Stalled ribosome:nascent molecule of interest complexes and methods of using same are provided. Plasmids, particularly DNA plasmids, comprising a stall segment are also disclosed. The methods provide for the facile and stable formation of stalled ribosome:nascent molecule of interest complexes that may be used to examine protein synthesis and protein conformational events, as well as in the creation of desired ribosomal displays. Cells transformed with these plasmids are also provided, and include both eukaryotic and prokaryotic transformed cells. Stall elements that provide for ribosomal stalling of eukaryotic and prokaryotic ribosomes are also disclosed. Various therapeutic and clinical applications of these methods are also provided and used in diseases associated with defects in protein accumulation in vivo.


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