The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 14, 2015
Filed:
Sep. 01, 2010
Shinya Yamanaka, Kyoto, JP;
Takayuki Tanaka, Kyoto, JP;
Shinya Yamanaka, Kyoto, JP;
Takayuki Tanaka, Kyoto, JP;
Kyoto University, Kyoto, JP;
Abstract
The present invention relates to a method for selecting induced pluripotent stem (iPS) cells. More particularly, the present invention provides: a method for selecting an iPS cell, comprising the steps of: (1a) measuring the expression level of an exogenous nuclear reprogramming gene(s) in a test iPS cell; and (2a) selecting an iPS cell in which the expression level(s) of an exogenous nuclear reprogramming gene(s) is/are less than or equal to the expression level(s) in control iPS cells; and a method for selecting an iPS cell, comprising the steps of: (1b) measuring the expression level of an exogenous nuclear reprogramming gene(s) and the sum the expression levels of the exogenous nuclear reprogramming gene(s) and the corresponding endogenous gene iPS cell; and (2b) selecting an iPS cell in which the ratio of the expression level of an exogenous nuclear reprogramming gene(s) relative to the sum of the expression levels of the exogenous transgene(s) and the corresponding endogenous gene(s) is less than 1 to the ratio in the control iPS cell.