The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2015

Filed:

Sep. 16, 2011
Applicants:

Ellen Burke, San Diego, CA (US);

Steven John Gort, Apple Valley, MN (US);

Paula M. Hicks, Bend, OR (US);

Peter Luginbuhl, San Diego, CA (US);

Sara C. Mcfarlan, St. Paul, MN (US);

Toby Richardson, San Diego, CA (US);

Christopher Solheid, Minneapolis, MN (US);

David Weiner, Del Mar, CA (US);

Lishan Zhao, Emeryville, CA (US);

Inventors:

Ellen Burke, San Diego, CA (US);

Steven John Gort, Apple Valley, MN (US);

Paula M. Hicks, Bend, OR (US);

Peter Luginbuhl, San Diego, CA (US);

Sara C. McFarlan, St. Paul, MN (US);

Toby Richardson, San Diego, CA (US);

Christopher Solheid, Minneapolis, MN (US);

David Weiner, Del Mar, CA (US);

Lishan Zhao, Emeryville, CA (US);

Assignee:

Cargill, Incorporated, Wayzata, MN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/10 (2006.01); C12P 13/22 (2006.01); C12N 9/10 (2006.01); C12P 21/06 (2006.01); C12P 19/34 (2006.01); C07H 21/04 (2006.01); C12N 9/88 (2006.01); C12P 13/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/88 (2013.01); C12P 13/001 (2013.01); C12P 17/10 (2013.01);
Abstract

This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.


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