The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2015
Filed:
Sep. 06, 2012
David M. Donovan, Baltimore, MD (US);
Igor V. Abaev, Obolensk Moscow Region, RU;
Mathias Schmelcher, Gockhausen, CH;
David M. Donovan, Baltimore, MD (US);
Igor V. Abaev, Obolensk Moscow Region, RU;
Mathias Schmelcher, Gockhausen, CH;
The United States of America, as represented by The Secretary of Agriculture, Washington, DC (US);
Abstract
Methicillin-resistant (MRSA) and multi-drug resistant strains ofare becoming increasingly prevalent in both human and veterinary clinics.-causing bovine mastitis yields high annual losses to the dairy industry. Treatment of mastitis by broad range antibiotics is often not successful and may contribute to development of antibiotic resistance. Bacteriophage endolysins are a promising new source of antimicrobials. The endolysin of prophage φSH2 ofstrain JCSC1435 (φSH2 lysin) shows lytic activity against live staphylococcal cells. Deletion constructs were tested in zymograms and turbidity reduction assays to evaluate the contribution of each functional module to lysis. The CHAP domain exhibited three-fold higher activity than the full length protein. Activity was further enhanced in the presence of bivalent calcium ions. The full length enzyme and the CHAP domain showed activity against multiple staphylococcal strains, including MRSA strains, mastitis isolates, and coagulase negative staphylococcal (CoNS) strains.