The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

Jul. 22, 2009
Applicants:

Hitto Kaufmann, Ulm, DE;

Lore Florin, Biberach, DE;

Eric Becker, Hochdorf, DE;

Joey M. Studts, Biberach, DE;

Inventors:

Hitto Kaufmann, Ulm, DE;

Lore Florin, Biberach, DE;

Eric Becker, Hochdorf, DE;

Joey M. Studts, Biberach, DE;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12P 21/02 (2006.01); C07K 16/00 (2006.01); C12N 9/06 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 16/00 (2013.01); C12N 9/003 (2013.01);
Abstract

The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper® Discovery) and DSM ACC2910 (CHOpper® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.


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