The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

Nov. 15, 2011
Applicants:

Eva Albers, Partille, SE;

Lisbeth Olsson, Göteborg, SE;

Rakesh Koppram, Göteborg, SE;

Inventors:

Eva Albers, Partille, SE;

Lisbeth Olsson, Göteborg, SE;

Rakesh Koppram, Göteborg, SE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/12 (2006.01); C12P 7/06 (2006.01); C12P 7/10 (2006.01); C12R 1/865 (2006.01); C12N 9/04 (2006.01); C12N 15/01 (2006.01); C12N 15/81 (2006.01); C12Q 1/04 (2006.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
C12P 7/06 (2013.01); C12P 7/10 (2013.01); C12R 1/865 (2013.01); C12N 9/0006 (2013.01); C12N 15/01 (2013.01); C12N 15/81 (2013.01); C12Q 1/04 (2013.01); C12N 9/1205 (2013.01); C12Y 101/01009 (2013.01); C12Y 101/0101 (2013.01); C12Y 101/01307 (2013.01); C12Y 207/01017 (2013.01); Y02E 50/16 (2013.01); Y02E 50/17 (2013.01);
Abstract

A method for producing a strain ofwith introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain ofat a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains ofobtained by the method according to the invention are described.


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