The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

May. 09, 2008
Applicants:

David J. Reczek, Sudbury, MA (US);

Paul Saftig, Gettorf, DE;

Christine T. Demaria, Franklin, MA (US);

Inventors:

David J. Reczek, Sudbury, MA (US);

Paul Saftig, Gettorf, DE;

Christine T. DeMaria, Franklin, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 9/24 (2006.01); C12N 15/00 (2006.01); C12N 5/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12P 21/02 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C12N 15/1138 (2013.01); C12Y 302/01045 (2013.01); C12N 9/2402 (2013.01); C12N 2310/14 (2013.01);
Abstract

The invention is directed to methods of producing a polypeptide or a variant thereof, wherein the polypeptide or variant thereof is dependent on LIMP-2 for trafficking, localization, stabilization and/or sorting of the polypeptide in the cell. In general, the methods comprise culturing a lysosomal integral membrane protein II (LIMP-2) deficient cell which expresses the polypeptide or the variant thereof under conditions in which the polypeptide or the variant thereof is produced.


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