The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 17, 2015

Filed:

Apr. 27, 2008
Applicants:

Masha Idelson, MaAle Adumim, IL;

Ruslana Alper-pinus, Jerusalem, IL;

Alex Obolensky, Jerusalem, IL;

Eyal Banin, Jerusalem, IL;

Benjamin Reubinoff, Moshav Bar-Giora-Doar-Na HaEla, IL;

Inventors:

Masha Idelson, MaAle Adumim, IL;

Ruslana Alper-Pinus, Jerusalem, IL;

Alex Obolensky, Jerusalem, IL;

Eyal Banin, Jerusalem, IL;

Benjamin Reubinoff, Moshav Bar-Giora-Doar-Na HaEla, IL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 5/079 (2010.01); A61K 35/44 (2006.01); A61K 35/48 (2006.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); C12N 2501/115 (2013.01); C12N 2500/99 (2013.01); C12N 5/0696 (2013.01); C12N 2533/52 (2013.01); C12N 2502/13 (2013.01); A61K 35/44 (2013.01); A61K 35/48 (2013.01); C12N 2500/38 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2506/02 (2013.01);
Abstract

The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.


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