The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 17, 2015
Filed:
Nov. 10, 2010
Maria Chiara Bonini, Peschiera Borromeo, IT;
Pietro Genovese, Messina, IT;
Philip D. Gregory, Orinda, CA (US);
Michael C. Holmes, Oakland, CA (US);
Luigi Naldini, Pioltello, IT;
David Paschon, Oakland, CA (US);
Elena Provasi, Cislago, IT;
Lei Zhang, Davis, CA (US);
Maria Chiara Bonini, Peschiera Borromeo, IT;
Pietro Genovese, Messina, IT;
Philip D. Gregory, Orinda, CA (US);
Michael C. Holmes, Oakland, CA (US);
Luigi Naldini, Pioltello, IT;
David Paschon, Oakland, CA (US);
Elena Provasi, Cislago, IT;
Lei Zhang, Davis, CA (US);
Sangamo BioSciences, Inc., Richmond, CA (US);
Ospedale San Raffaele S.R.L., Milan, IT;
Abstract
Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.