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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 8932828 B1

PDFFull Text
Date of Patent:
Jan. 13, 2015

Filed:

Sep. 29, 2010

Method for preparing recombinant granulocyte colony stimulating factor cysteine muteins

Applicants:

Mary S. Rosendahl, Broomfield, CO (US);

George N. Cox, Louisville, CO (US);

Daniel H. Doherty, Boulder, CO (US);

Inventors:

Mary S. Rosendahl, Broomfield, CO (US);

George N. Cox, Louisville, CO (US);

Daniel H. Doherty, Boulder, CO (US);

Assignee:

Bolder Biotechnology, Inc., Boulder, CO (US);

Attorney:

Sheridan Ross P.C.

Primary Examiner:

Bridget E Bunner

Assistant Examiner:

Christina Borgeest

Int. Cl.
CPC ...
C12P 21/00 (2006.01); C07K 1/00 (2006.01); C07K 1/14 (2006.01); C07K 1/16 (2006.01); C07K 2/00 (2006.01); C07K 14/515 (2006.01); C07K 1/113 (2006.01); C07K 14/505 (2006.01); C07K 14/56 (2006.01); C07K 14/535 (2006.01); C07K 14/52 (2006.01); C07K 14/61 (2006.01); A61K 47/48 (2006.01); C07K 14/78 (2006.01);
U.S. Cl.
CPC ...
C07K 14/52 (2013.01); C07K 14/515 (2013.01); C07K 1/1133 (2013.01); C07K 14/505 (2013.01); C07K 14/56 (2013.01); C07K 14/535 (2013.01); C07K 14/61 (2013.01); A61K 47/48215 (2013.01); C07K 14/78 (2013.01);
Abstract

The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.

Find Patent Forward Citations

Rosendahl, Mary. (2015). Method for preparing recombinant granulocyte colony stimulating factor cysteine muteins (U.S. Patent No. 8932828). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/8932828

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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