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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 8916540 B1

PDFFull Text
Date of Patent:
Dec. 23, 2014

Filed:

Jan. 15, 2009

Antibiotic compositions and related screening methods

Applicants:

Chi-huey Wong, La Jolla, CA (US);

Ting-jen Cheng, Taipei, TW;

Che Alex MA, Taipei, TW;

Wei-chieh Cheng, Taipei, TW;

Inventors:

Chi-Huey Wong, La Jolla, CA (US);

Ting-Jen Cheng, Taipei, TW;

Che Alex Ma, Taipei, TW;

Wei-Chieh Cheng, Taipei, TW;

Assignee:

Academia Sinica, Taipei, TW;

Attorney:

Eckman Basu LLP

Primary Examiner:

Eric Olson

Int. Cl.
CPC ...
C07K 14/195 (2006.01); C07K 14/26 (2006.01); C07K 14/315 (2006.01); C07K 14/25 (2006.01); C07K 14/235 (2006.01); C07K 14/32 (2006.01); C07K 14/31 (2006.01); C07K 14/21 (2006.01); C07K 14/33 (2006.01); C07K 14/255 (2006.01); C07K 14/22 (2006.01); C07K 14/265 (2006.01); G01N 33/573 (2006.01); A61K 31/19 (2006.01); A61K 31/715 (2006.01); A61K 31/427 (2006.01); A61K 31/365 (2006.01);
U.S. Cl.
CPC ...
G01N 33/573 (2013.01); C07K 14/3156 (2013.01); C07K 14/32 (2013.01); C07K 14/21 (2013.01); C07K 14/31 (2013.01); C07K 14/26 (2013.01); C07K 14/235 (2013.01); C07K 14/315 (2013.01); C07K 14/33 (2013.01); A61K 31/19 (2013.01); C07K 14/195 (2013.01); A61K 31/715 (2013.01); C07K 14/25 (2013.01); A61K 31/427 (2013.01); A61K 31/365 (2013.01); C07K 14/265 (2013.01); C07K 14/22 (2013.01); G01N 2333/91091 (2013.01); C07K 14/255 (2013.01);
Abstract

Moenomycin inhibits bacterial growth by clocking the transglycosylase activity of class A penicillin-binding proteins (PBPs), which are key enzymes in bacterial cell wall synthesis. The binding affinities of moenomycin A with various truncated PBPs were compared showing that the transmembrane domain is important for moenomycin binding. Full-length class-A PBPs from 16 bacterial species were produced, and their binding activities showed a correlation with the antimicrobial activity of moenomycin againstand. Moreover, a fluorescence anisotropy-based high-throughput assay was developed and used successfully for identification of transglycosylase inhibitors.

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