The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 2014
Filed:
Oct. 24, 2008
Christof Fellmann, Cold Spring Harbor, NY (US);
Scott W. Lowe, Cold Spring Harbor, NY (US);
Gregory J. Hannon, Huntington, NY (US);
Johannes E. Zuber, Cold Spring Harbor, NY (US);
Christof Fellmann, Cold Spring Harbor, NY (US);
Scott W. Lowe, Cold Spring Harbor, NY (US);
Gregory J. Hannon, Huntington, NY (US);
Johannes E. Zuber, Cold Spring Harbor, NY (US);
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (US);
Abstract
Provided is a single construct combining a sequence encoding an RNAi molecule, a sequence encoding a reporter, and a target sequence specific for the RNAi molecule. The construct can be used to determine the potency of the encoded RNAi molecule in a direct and unbiased way. These results can be used to inform the design of potent RNAi molecules of various types and can be extended to several other applications, including: (1) generation of tiled libraries comprising every possible RNAi molecule-encoding sequence for a given gene target; (2) large-scale parallel validation of RNAi molecules targeting many genes to generate validated RNAi molecule-encoding libraries; (3) experimental comparison of design algorithms and strategies; and (4) investigation of RNAi biology in target site mutagenesis assays by screening pools containing single nucleotide changes in target sites and/or in the RNAi molecule to identify the most relevant sequence characteristics of potent RNAi-target site predictions.