The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 2014
Filed:
Feb. 08, 2012
Applicants:
Andrey Shaw, St. Louis, MO (US);
Jiancheng HU, St. Louis, MO (US);
Haiyang Yu, St. Louis, MO (US);
Inventors:
Assignee:
Washington University, Saint Louis, MO (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0069 (2013.01); C12N 9/1205 (2013.01); C07K 2319/70 (2013.01); G01N 2333/90241 (2013.01); G01N 2333/9121 (2013.01);
Abstract
Mutant KSR proteins are disclosed. The mutants include single amino acid substitutions, leading to either a loss of kinase activity or a loss of scaffolding activity. Also disclosed are methods of screening compounds for inhibitors of KSR kinase activity or KSR scaffolding activity. In some embodiments, the screening methods include protein complementation assays in which nucleic acids encoding fusion constructs comprising enzyme portions and kinase dimerization domains are expressed in cells. Inhibitors of dimerization can be indicated by loss of enzyme activity.