The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 25, 2014

Filed:

May. 07, 2009
Applicants:

Elizabeth M. Simpson, Vancouver, CA;

Wyeth W. Wasserman, Vancouver, CA;

Robert A. Holt, Vancouver, CA;

Steven J. Jones, Vancouver, CA;

Daniel Goldowitz, Vancouver, CA;

Elodie Portales-casamar, Vancouver, CA;

Cletus D'souza, Vancouver, CA;

Vikramjit Chopra, Vancouver, CA;

Inventors:

Elizabeth M. Simpson, Vancouver, CA;

Wyeth W. Wasserman, Vancouver, CA;

Robert A. Holt, Vancouver, CA;

Steven J. Jones, Vancouver, CA;

Daniel Goldowitz, Vancouver, CA;

Elodie Portales-Casamar, Vancouver, CA;

Cletus D'Souza, Vancouver, CA;

Vikramjit Chopra, Vancouver, CA;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/00 (2006.01); C07H 21/02 (2006.01); C12N 15/00 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 15/85 (2006.01); C07K 14/47 (2006.01);
U.S. Cl.
CPC ...
C07K 14/47 (2013.01); C12N 2800/00 (2013.01); C12N 2830/008 (2013.01); C12N 15/85 (2013.01);
Abstract

Isolated polynucleotides comprising an S100B promoter are provided, where an S100B regulatory element is operably joined to an S100B basal promoter utilizing a non-native spacing between the promoter and regulatory elements. The promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, etc.


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