The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 18, 2014
Filed:
Sep. 13, 2012
3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Brian Stupi, Cypress, TX (US);
Hong LI, Milwaukee, WI (US);
Weidong Wu, Houston, TX (US);
Megan N. Hersh, Houston, TX (US);
David Hertzog, Houston, TX (US);
Sidney E. Morris, Houston, TX (US);
Michael L. Metzker, Houston, TX (US);
Brian Stupi, Cypress, TX (US);
Hong Li, Milwaukee, WI (US);
Weidong Wu, Houston, TX (US);
Megan N. Hersh, Houston, TX (US);
David Hertzog, Houston, TX (US);
Sidney E. Morris, Houston, TX (US);
Michael L. Metzker, Houston, TX (US);
Lasergen, Inc., Houston, TX (US);
Abstract
The present invention relates generally to 3'-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas: wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.