The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 18, 2014
Filed:
Jan. 20, 2011
Emil P. Kartalov, Pasadena, CA (US);
Darryl Shibata, Rolling Hills Estates, CA (US);
Clive Taylor, Malibu, CA (US);
Lawrence A. Wade, La Canada-Flintridge, CA (US);
Emil P. Kartalov, Pasadena, CA (US);
Darryl Shibata, Rolling Hills Estates, CA (US);
Clive Taylor, Malibu, CA (US);
Lawrence A. Wade, La Canada-Flintridge, CA (US);
California Institute of Technology, Pasadena, CA (US);
University of Southern California, Los Angeles, CA (US);
Abstract
Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.