The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 04, 2014
Filed:
Jun. 15, 2012
Seung Goo Lee, Daejeon, KR;
Su-lim Choi, Daejeon, KR;
Jong Sik Gam, Daejeon, KR;
Jae Jun Song, Daejeon, KR;
Sang Jun Lee, Daejeon, KR;
Seung Goo Lee, Daejeon, KR;
Su-Lim Choi, Daejeon, KR;
Jong Sik Gam, Daejeon, KR;
Jae Jun Song, Daejeon, KR;
Sang Jun Lee, Daejeon, KR;
Korea Research Institute of Bioscience and Biotechnology, Daejeon, KR;
Abstract
The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.