The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 28, 2014
Filed:
Sep. 16, 2010
Marie Cellier, Montalieu Vercieu, FR;
John Mills, Fenton, MO (US);
David Mosticone, Sainte Consorce, FR;
Sylvain Orenga, Neuville sur Ain, FR;
Antoine Vimont, Lyons, FR;
Marie Cellier, Montalieu Vercieu, FR;
John Mills, Fenton, MO (US);
David Mosticone, Sainte Consorce, FR;
Sylvain Orenga, Neuville sur Ain, FR;
Antoine Vimont, Lyons, FR;
bioMérieux, S.A., Marcy L'Etoile, unknown;
Abstract
The present invention relates to a method for identifying bacteria of thegroup, comprising the following steps: (a) providing a sample that may contain bacteria of thegroup, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of thegroup by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of thegroup is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to thegroup, that may be present in the sample.