The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 23, 2014
Filed:
Mar. 06, 2009
Nobuyuki Kurosawa, Toyama, JP;
Masaharu Isobe, Toyama, JP;
Nobuyuki Kurosawa, Toyama, JP;
Masaharu Isobe, Toyama, JP;
National University Corporation University of Toyama, Toyama-shi, JP;
Abstract
The present invention provides a method which can achieve the homologous recombination of a gene of interest selectively, and a recombinant DNA molecule produced by the method. A homologous recombination method which uses a PCR product and a linearized vector is disclosed. The PCR product comprises a sequence for a target gene and amplification primer sequences P1 and P2 on both terminal ends. The vector contains homologous recombination regions VP1 and VP2 which respectively comprise nucleotide sequences homologous to P1 and P2, and at least one a homologous recombination region VT (VT1 and/or VT2), which comprises a nucleotide sequence homologous to a sequence T (T1 and/or T2), T sequences are sequence parts internal to P1 and/or P2 as well as sequence parts on the terminal side of VP1 and/or VP2 (provided that at least one T sequence has a nucleotide sequence specific to the target gene).