The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 16, 2014
Filed:
Feb. 28, 2006
Michael Seibert, Lakewood, CO (US);
Paul W. King, Golden, CO (US);
Maria Lucia Ghirardi, Lakewood, CO (US);
Matthew C. Posewitz, Golden, CO (US);
Sharon L. Smolinski, Littleton, CO (US);
Michael Seibert, Lakewood, CO (US);
Paul W. King, Golden, CO (US);
Maria Lucia Ghirardi, Lakewood, CO (US);
Matthew C. Posewitz, Golden, CO (US);
Sharon L. Smolinski, Littleton, CO (US);
Alliance for Sustainable Energy, LLC, Golden, CO (US);
Abstract
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.