The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2014
Filed:
Dec. 14, 2011
Subhayu Basu, Chestnut Hill, MA (US);
Gaozhong Shen, Brighton, MA (US);
Brett Boghigian, Boston, MA (US);
David Young, Cambridge, MA (US);
David Berry, Cambridge, MA (US);
Geoffrey Von Maltzahn, Cambridge, MA (US);
Subhayu Basu, Chestnut Hill, MA (US);
Gaozhong Shen, Brighton, MA (US);
Brett Boghigian, Boston, MA (US);
David Young, Cambridge, MA (US);
David Berry, Cambridge, MA (US);
Geoffrey von Maltzahn, Cambridge, MA (US);
Pronutria, Inc., Cambridge, MA (US);
Abstract
Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and α-linolenic acid.