The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2014
Filed:
Jul. 03, 2008
James J. Cali, Verona, WI (US);
Dieter Klaubert, Arroyo Grande, CA (US);
William Daily, Santa Maria, CA (US);
Samuel K. Ho, New Bedford, MA (US);
Susan Frackman, Madison, WI (US);
Erika Hawkins, Pembroke, CA;
Keith V. Wood, Mt. Horeb, WI (US);
James J. Cali, Verona, WI (US);
Dieter Klaubert, Arroyo Grande, CA (US);
William Daily, Santa Maria, CA (US);
Samuel K. Ho, New Bedford, MA (US);
Susan Frackman, Madison, WI (US);
Erika Hawkins, Pembroke, CA;
Keith V. Wood, Mt. Horeb, WI (US);
Promega Corporation, Madison, WI (US);
Abstract
The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.