The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 03, 2014
Filed:
Apr. 21, 2010
Applicants:
Hisahiro Tabuchi, Tokyo, JP;
Tomoya Sugiyama, Tokyo, JP;
Inventors:
Hisahiro Tabuchi, Tokyo, JP;
Tomoya Sugiyama, Tokyo, JP;
Assignee:
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/82 (2006.01); C12N 15/74 (2006.01); C12N 1/19 (2006.01); C12N 5/02 (2006.01);
U.S. Cl.
CPC ...
Abstract
The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly bicarbonate transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.