The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 27, 2014
Filed:
Mar. 18, 2010
Shigeyuki Yokoyama, Kanagawa, JP;
Kensaku Sakamoto, Kanagawa, JP;
Tatsuo Yanagisawa, Kanagawa, JP;
Takatsugu Kobayashi, Kanagawa, JP;
Shigeyuki Yokoyama, Kanagawa, JP;
Kensaku Sakamoto, Kanagawa, JP;
Tatsuo Yanagisawa, Kanagawa, JP;
Takatsugu Kobayashi, Kanagawa, JP;
Riken, Wako-Shi, JP;
Abstract
Method for incorporating a lysine derivative (particularly an N-benzyloxycarbonyl-lysine (Z-Lys) derivative) having useful functional group such as heavy atom, selenium, reactive functional group, fluorescent group or crosslinker, which is suitable as a non-natural amino acid, into a desired protein in a site-specific manner. A mutant pyrrolysyl-tRNA synthetase has substitution of at least one amino acid residue selected from tyrosine residue at position 306, leucine residue at position 309 and cysteine residue at position 348 each constituting a pyrrolysine-binding site in the amino acid sequence for pyrrolysyl-tRNA synthetase of SEQ ID NO:2. The substitution of the amino acid residue is: of tyrosine residue at position 306 by glycine or alanine residue, of leucine residue at position 309 by glycine or alanine residue, and/or of a cysteine residue at position 348 by valine, serine or alanine residue.