The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 27, 2014
Filed:
Mar. 14, 2013
Jones M. Hyman, Wake Forest, NC (US);
Parampal Deol, Raleigh, NC (US);
Elizabeth Miller, Wake Forest, NC (US);
Victoria Girard, Lyons, FR;
Amber Gates, Durham, NC (US);
Sandrine Mailler, Courtenay, FR;
Maud Arsac, Saint Chamond, FR;
John Walsh, Durham, NC (US);
Jones M. Hyman, Wake Forest, NC (US);
Parampal Deol, Raleigh, NC (US);
Elizabeth Miller, Wake Forest, NC (US);
Victoria Girard, Lyons, FR;
Amber Gates, Durham, NC (US);
Sandrine Mailler, Courtenay, FR;
Maud Arsac, Saint Chamond, FR;
John Walsh, Durham, NC (US);
BioMerieux, Inc., Durham, NC (US);
Abstract
The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g.,or), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.