The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 08, 2014

Filed:

Jul. 15, 2010
Applicants:

Yoshihisa Nakano, Osaka, JP;

Mitsuhiro Uenoyama, Osaka, JP;

Erina Kobayashi, Osaka, JP;

Inventors:

Yoshihisa Nakano, Osaka, JP;

Mitsuhiro Uenoyama, Osaka, JP;

Erina Kobayashi, Osaka, JP;

Assignees:

Bio-I Co., Ltd., Osaka, JP;

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/14 (2006.01); C12N 9/88 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by anfilamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity. It is possible to inexpensively mass produce food products which retain water-soluble components within the cell, because physical treatment such as stirring or beating is also unnecessary.


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