The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2014
Filed:
Sep. 30, 2005
Matthias Ballhause, Berlin, DE;
Kurt Berlin, Stahnsdorf, DE;
Dimo Dietrich, Berlin, DE;
Antje Kluth Lukas, Wentorf, DE;
Matthias Schuster, Berlin, DE;
Ute Wagner, Berlin, DE;
Reinhold Wasserkort, Berlin, DE;
Heike Ziebarth, Berlin, DE;
Matthias Ballhause, Berlin, DE;
Kurt Berlin, Stahnsdorf, DE;
Dimo Dietrich, Berlin, DE;
Antje Kluth Lukas, Wentorf, DE;
Matthias Schuster, Berlin, DE;
Ute Wagner, Berlin, DE;
Reinhold Wasserkort, Berlin, DE;
Heike Ziebarth, Berlin, DE;
Epigenomics AG, Berlin, DE;
Abstract
Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.