The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2014
Filed:
Sep. 17, 2004
Mitsufumi Wade, Mobara, JP;
Toshihiro Oikawa, Mobara, JP;
Daisuke Mochizuki, Mobara, JP;
Junko Tokuda, Mobara, JP;
Miyuki Kawashima, Mobara, JP;
Tadashi Araki, Mobara, JP;
Reiko Abe, Mobara, JP;
Hitoki Miyake, Mobara, JP;
Hitoshi Takahashi, Mobara, JP;
Hideki Sawai, Kamakura, JP;
Takashi Mimitsuka, Kamakura, JP;
Takashi Morishige, Mobara, JP;
Yosuke Higashi, Mobara, JP;
Mitsufumi Wade, Mobara, JP;
Toshihiro Oikawa, Mobara, JP;
Daisuke Mochizuki, Mobara, JP;
Junko Tokuda, Mobara, JP;
Miyuki Kawashima, Mobara, JP;
Tadashi Araki, Mobara, JP;
Reiko Abe, Mobara, JP;
Hitoki Miyake, Mobara, JP;
Hitoshi Takahashi, Mobara, JP;
Hideki Sawai, Kamakura, JP;
Takashi Mimitsuka, Kamakura, JP;
Takashi Morishige, Mobara, JP;
Yosuke Higashi, Mobara, JP;
Mitsui Chemicals, Inc., Minato-Ku, Tokyo, JP;
Abstract
A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.