The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 25, 2014

Filed:

Sep. 08, 2010
Applicants:

Keiko Abe, Tokyo, JP;

Takumi Misaka, Tokyo, JP;

Takamasa Imada, Tokyo, JP;

Satoshi Fujiwara, Kawasaki, JP;

Inventors:

Keiko Abe, Tokyo, JP;

Takumi Misaka, Tokyo, JP;

Takamasa Imada, Tokyo, JP;

Satoshi Fujiwara, Kawasaki, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); G01N 33/567 (2006.01); C07K 14/705 (2006.01);
U.S. Cl.
CPC ...
Abstract

[Object] An object of the present invention is to provide a sweet taste receptor-expressing construct which can functionally stably express both of a sweet taste receptor (T1R2+T1R3) and a G protein α subunit at a high expression efficacy, and a stable expression cell body expressing the construct. [Solution] The sweet taste receptor-expressing construct of the present invention is such that respective genes encoding sweet taste receptor subunits T1R2 and T1R3, and a G protein α subunit are inserted into the same plasmid. The cell body of the present invention is such that the sweet taste receptor-expressing construct of the present invention is gene-introduced into a 293 cell in which an FRT (Flippase Recognition Target) sequence is incorporated into one place in a genome, to express sweet taste receptor subunits T1R2 and T1R3, and a G protein α subunit simultaneously.


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