The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 11, 2014

Filed:

Jun. 08, 2010
Applicants:

Seung Goo Lee, Daejeon, KR;

Su Lim Choi, Daejeon, KR;

Eugene Rha, Daejeon, KR;

Jae Jun Song, Daejeon, KR;

Inventors:

Seung Goo Lee, Daejeon, KR;

Su Lim Choi, Daejeon, KR;

Eugene Rha, Daejeon, KR;

Jae Jun Song, Daejeon, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.


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