The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2014
Filed:
Jun. 21, 2010
Yasuhiro Kajihara, Toyonaka, JP;
Ryo Okamoto, Nishitokyo, JP;
Izumi Sakamoto, Tokushima, JP;
Kazuyuki Ishii, Tokyo, JP;
Yasuhiro Kajihara, Toyonaka, JP;
Ryo Okamoto, Nishitokyo, JP;
Izumi Sakamoto, Tokushima, JP;
Kazuyuki Ishii, Tokyo, JP;
Glytech Inc., Kyoto, JP;
Abstract
A process for chemically converting a peptide chain into a peptide thioester includes, when a —C(═X)—Rgroup is introduced to the thiol group of the cysteine residue and then the resulting peptide is reacted with a compound having a leaving group represented by the formula: —NH—C(═Y)NHRin an organic solvent, the —NH—C(═Y)NHRgroup binds via addition reaction to the carboxyl group of the N-terminal-side peptide bond of the cysteine residue, whereby the peptide bond is cleaved and the C-terminal-side peptide fragment is cut off. Further, when the resulting peptide chain having the —NH—C(═Y)NHRgroup is reacted with a thiol in a buffer solution, a thiol exchange reaction occurs, namely, the thiol group of the thiol binds to the carbonyl carbon to which the —NH—C(═Y)NHRgroup has bound, whereby the —NH—C(═Y)NHRgroup is eliminated.