The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 07, 2014
Filed:
May. 31, 2012
Sylvain Arnould, Paris, FR;
Patrick Chames, Paris, FR;
Phillippe Duchateau, Cerny, FR;
Jean-charles Epinat, Paris, FR;
Emmanuel Lacroix, Paris, FR;
Frederic Paques, Bourg la Reine, FR;
Sylvain Arnould, Paris, FR;
Patrick Chames, Paris, FR;
Phillippe Duchateau, Cerny, FR;
Jean-Charles Epinat, Paris, FR;
Emmanuel Lacroix, Paris, FR;
Frederic Paques, Bourg la Reine, FR;
Cellectis S.A., Paris, FR;
Abstract
A monomer of an I-CreI meganuclease variant wherein said monomer comprises mutations in the amino acid sequence of SEQ ID NO: 34, wherein said mutations include, (i) at least one and up to five amino acid substitutions from residue Q44 to residue R70, said substitutions selected from the group consisting of substitutions at positions Q44, T46, Y66, R68 and R7; and (ii) at least one and up to six amino acid substitutions from residue Q26 to residue Q38 said substitutions selected from the group consisting of substitutions at positions Q26, K28, N30, S32, Y33 and Q38, and wherein said monomer when in dimeric form binds and cleaves a DNA target sequence. Said dimeric forms include homodimeric, heterodimeric and single-chain I-CreI meganuclease variants.