The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 2013
Filed:
Sep. 09, 2009
Lore Florin, Biberach, DE;
Rebecca Bischoff, Warthausen, DE;
Juergen Fieder, Biberach, DE;
Hitto Kaufmann, Ulm, DE;
Thomas Krieg, Warthausen, DE;
Lore Florin, Biberach, DE;
Rebecca Bischoff, Warthausen, DE;
Juergen Fieder, Biberach, DE;
Hitto Kaufmann, Ulm, DE;
Thomas Krieg, Warthausen, DE;
Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, DE;
Abstract
The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.