The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 22, 2013
Filed:
Mar. 16, 2009
Jüergen Fieder, Biberach, DE;
Lore Florin, Biberach, DE;
Hitto Kaufmann, Ulm, DE;
Thomas Krieg, Warthausen, DE;
Melanie Briegel, Altheim, DE;
Jüergen Fieder, Biberach, DE;
Lore Florin, Biberach, DE;
Hitto Kaufmann, Ulm, DE;
Thomas Krieg, Warthausen, DE;
Melanie Briegel, Altheim, DE;
Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, DE;
Abstract
The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells. By using IGF particularly in conjunction with HSA in the culture medium after recloning, the recloning efficiency and hence the quantity of clones obtained can be increased significantly.