The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2013
Filed:
Jun. 03, 2011
Sylvain Arnould, Paris, FR;
Patrick Chames, Paris, FR;
Philippe Duchateau, Cerny, FR;
Jean-charles Epinat, Paris, FR;
Emmanuel Lacroix, Paris, FR;
Frederic Paques, Bourg la Reine, FR;
Sylvain Arnould, Paris, FR;
Patrick Chames, Paris, FR;
Philippe Duchateau, Cerny, FR;
Jean-Charles Epinat, Paris, FR;
Emmanuel Lacroix, Paris, FR;
Frederic Paques, Bourg la Reine, FR;
Cellectis S.A., Paris, FR;
Abstract
The present invention relates to a polynucleotide encoding a monomer of an I-CreI variant said monomer comprising mutations in the amino acid sequence of SEQ ID NO: 34, wherein said mutations include: (i) at least one and up to eleven amino acid substitutions from residue S22 to Q44 said substitutions selected from the group consisting of substitutions at positions I24, Q26, K28, N30, S32, Y33, Q38, S40 and T42; and (ii) at least one and up to six amino acid substitutions from residue Y66 to I77 said substitutions selected from the group consisting of substitutions at positions Y66, R68, R70, V73 and I77; and wherein said monomer when in dimeric form binds and cleaves DNA.