The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 13, 2013
Filed:
Jun. 26, 2012
Peter A. Carr, Medford, MA (US);
Brian Y. Chow, Cambridge, MA (US);
Joseph M. Jacobson, Newton, MA (US);
David W. Mosley, Cambridge, MA (US);
Christopher Emig, Napa, CA (US);
Peter A. Carr, Medford, MA (US);
Brian Y. Chow, Cambridge, MA (US);
Joseph M. Jacobson, Newton, MA (US);
David W. Mosley, Cambridge, MA (US);
Christopher Emig, Napa, CA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Abstract
In a method for synthesizing a pool of nucleic acid molecules, a first nucleic acid has a first 5' region and a first 3′ region and a second nucleic acid has a second 5′ region and a second 3′ region. The second 3′ region and the first 5′ region have identical nucleic acid sequences. The first 3′ region is hybridized with an oligonucleotide, extending the hybridized oligonucleotide and producing a first extension product having a 3′ region complementary to the first 5′ region. The second nucleic acid is hybridized with the first extension product to hybridize the 3′ region of the first extension product to the second 3′ region, extending the 3′ region of the first extension product and producing a second extension product having a 3′ region complementary to the second 5′ region. Error-containing molecules are separated from error-free molecules by a component that selects for a sequence error.